phospho cdk mapk substrates Search Results


phospho mapk substrate motif antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc phospho mapk substrate motif antibody
<t>Phospho-MAPK</t> substrate proteomic screen. A, schematic representation of phospho-MAPK substrate proteomic screen in skeletal muscle. B, phospho-MAPK substrate proteomic analysis of differentially phosphorylated proteins in skeletal muscle of mkp-5+/+ and mkp-5−/− mice. Upper graphs show the log2-transformed values for the ratio of each phosphopeptide in mkp-5+/+ (WT) and mkp-5−/− (KO) skeletal muscles. ND, non-damaged muscle; CTX 2D, damaged muscle 2 days after cardiotoxin injection. The Venn diagrams represent the number of hyper- (red) and hypophosphorylated (green) proteins in mkp-5−/− skeletal muscles as compared with mkp-5+/+ muscles. Overlapping proteins represent those containing more than one phosphorylation site identified to be hyper- and hypophosphorylated. C, heat map of hyper- (red) and hypophosphorylated <t>(green)</t> <t>peptides</t> showing the ratio of differentially phosphorylated peptides in skeletal muscles between mkp-5+/+ and mkp-5−/− mice. The right panel indicates the ranking of hyperphosphorylated proteins based on their relative level of phosphorylation of each peptide compared between regenerating skeletal muscles of mkp-5+/+ and mkp-5−/− mice.
Phospho Mapk Substrate Motif Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho mapk substrate motif antibody/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
phospho mapk substrate motif antibody - by Bioz Stars, 2026-03
95/100 stars
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sarcoma htapp 951 smp 4652  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc sarcoma htapp 951 smp 4652
<t>Phospho-MAPK</t> substrate proteomic screen. A, schematic representation of phospho-MAPK substrate proteomic screen in skeletal muscle. B, phospho-MAPK substrate proteomic analysis of differentially phosphorylated proteins in skeletal muscle of mkp-5+/+ and mkp-5−/− mice. Upper graphs show the log2-transformed values for the ratio of each phosphopeptide in mkp-5+/+ (WT) and mkp-5−/− (KO) skeletal muscles. ND, non-damaged muscle; CTX 2D, damaged muscle 2 days after cardiotoxin injection. The Venn diagrams represent the number of hyper- (red) and hypophosphorylated (green) proteins in mkp-5−/− skeletal muscles as compared with mkp-5+/+ muscles. Overlapping proteins represent those containing more than one phosphorylation site identified to be hyper- and hypophosphorylated. C, heat map of hyper- (red) and hypophosphorylated <t>(green)</t> <t>peptides</t> showing the ratio of differentially phosphorylated peptides in skeletal muscles between mkp-5+/+ and mkp-5−/− mice. The right panel indicates the ranking of hyperphosphorylated proteins based on their relative level of phosphorylation of each peptide compared between regenerating skeletal muscles of mkp-5+/+ and mkp-5−/− mice.
Sarcoma Htapp 951 Smp 4652, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sarcoma htapp 951 smp 4652/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
sarcoma htapp 951 smp 4652 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


Phospho-MAPK substrate proteomic screen. A, schematic representation of phospho-MAPK substrate proteomic screen in skeletal muscle. B, phospho-MAPK substrate proteomic analysis of differentially phosphorylated proteins in skeletal muscle of mkp-5+/+ and mkp-5−/− mice. Upper graphs show the log2-transformed values for the ratio of each phosphopeptide in mkp-5+/+ (WT) and mkp-5−/− (KO) skeletal muscles. ND, non-damaged muscle; CTX 2D, damaged muscle 2 days after cardiotoxin injection. The Venn diagrams represent the number of hyper- (red) and hypophosphorylated (green) proteins in mkp-5−/− skeletal muscles as compared with mkp-5+/+ muscles. Overlapping proteins represent those containing more than one phosphorylation site identified to be hyper- and hypophosphorylated. C, heat map of hyper- (red) and hypophosphorylated (green) peptides showing the ratio of differentially phosphorylated peptides in skeletal muscles between mkp-5+/+ and mkp-5−/− mice. The right panel indicates the ranking of hyperphosphorylated proteins based on their relative level of phosphorylation of each peptide compared between regenerating skeletal muscles of mkp-5+/+ and mkp-5−/− mice.

Journal: The Journal of Biological Chemistry

Article Title: A Phosphoproteomic Screen Identifies a Guanine Nucleotide Exchange Factor for Rab3A Protein as a Mitogen-activated Protein (MAP) Kinase Phosphatase-5-regulated MAP Kinase Target in Interleukin 6 (IL-6) Secretion and Myogenesis *

doi: 10.1074/jbc.M116.769208

Figure Lengend Snippet: Phospho-MAPK substrate proteomic screen. A, schematic representation of phospho-MAPK substrate proteomic screen in skeletal muscle. B, phospho-MAPK substrate proteomic analysis of differentially phosphorylated proteins in skeletal muscle of mkp-5+/+ and mkp-5−/− mice. Upper graphs show the log2-transformed values for the ratio of each phosphopeptide in mkp-5+/+ (WT) and mkp-5−/− (KO) skeletal muscles. ND, non-damaged muscle; CTX 2D, damaged muscle 2 days after cardiotoxin injection. The Venn diagrams represent the number of hyper- (red) and hypophosphorylated (green) proteins in mkp-5−/− skeletal muscles as compared with mkp-5+/+ muscles. Overlapping proteins represent those containing more than one phosphorylation site identified to be hyper- and hypophosphorylated. C, heat map of hyper- (red) and hypophosphorylated (green) peptides showing the ratio of differentially phosphorylated peptides in skeletal muscles between mkp-5+/+ and mkp-5−/− mice. The right panel indicates the ranking of hyperphosphorylated proteins based on their relative level of phosphorylation of each peptide compared between regenerating skeletal muscles of mkp-5+/+ and mkp-5−/− mice.

Article Snippet: Lyophilized peptides were redissolved, and phosphopeptides were enriched by immunoprecipitation using a phospho-MAPK substrate motif antibody (2325, Cell Signaling Technology), which recognizes the conserved MAPK substrate motif (P X S*P or S*P X (R/K)).

Techniques: Transformation Assay, Injection

Validation of GRAB as a MKP-5-regulated MAPK substrate. A, FLAG-tagged GRAB was co-expressed with MKP-5 in 293 cells. Cells were serum-starved for 6 h and stimulated with 5 μm anisomycin (Aniso). Lysates were immunoblotted with pMAPK substrate (Sub) antibody and reprobed with anti-FLAG and anti-MKP-5 antibodies. B, FLAG-tagged GRAB was expressed in 293 cells and cells were serum-starved for 6 h and stimulated with 5 μm anisomycin. Whole cell lysates were transferred to membranes, and the blots were incubated with the indicated amount of purified MKP-5 protein for 3 h. Immunoblots were probed with phospho-p38 (pp38) MAPK or pMAPK substrate (Sub) antibodies and reprobed with p38 MAPK or FLAG antibody.

Journal: The Journal of Biological Chemistry

Article Title: A Phosphoproteomic Screen Identifies a Guanine Nucleotide Exchange Factor for Rab3A Protein as a Mitogen-activated Protein (MAP) Kinase Phosphatase-5-regulated MAP Kinase Target in Interleukin 6 (IL-6) Secretion and Myogenesis *

doi: 10.1074/jbc.M116.769208

Figure Lengend Snippet: Validation of GRAB as a MKP-5-regulated MAPK substrate. A, FLAG-tagged GRAB was co-expressed with MKP-5 in 293 cells. Cells were serum-starved for 6 h and stimulated with 5 μm anisomycin (Aniso). Lysates were immunoblotted with pMAPK substrate (Sub) antibody and reprobed with anti-FLAG and anti-MKP-5 antibodies. B, FLAG-tagged GRAB was expressed in 293 cells and cells were serum-starved for 6 h and stimulated with 5 μm anisomycin. Whole cell lysates were transferred to membranes, and the blots were incubated with the indicated amount of purified MKP-5 protein for 3 h. Immunoblots were probed with phospho-p38 (pp38) MAPK or pMAPK substrate (Sub) antibodies and reprobed with p38 MAPK or FLAG antibody.

Article Snippet: Lyophilized peptides were redissolved, and phosphopeptides were enriched by immunoprecipitation using a phospho-MAPK substrate motif antibody (2325, Cell Signaling Technology), which recognizes the conserved MAPK substrate motif (P X S*P or S*P X (R/K)).

Techniques: Incubation, Purification, Western Blot

Identification of phosphoserine 169 on GRAB as a major phosphorylation site. A, schematic representation of GRAB showing identified MAPK phosphorylation sites. CC, coiled coil. B, wild type GRAB or the indicated GRAB phosphorylation site mutants were co-expressed with vector alone or MKP-5 in 293 cells. Cells were serum-starved for 6 h and stimulated with 5 μm anisomycin (Aniso). Total cell lysates were immunoblotted with pMAPK substrate (Sub) antibody and reprobed with FLAG, MKP-5, or p38 MAPK antibody. C, 293 cells were transfected with FLAG-tagged GRAB, and cells were serum-starved for 6 h and stimulated with 5 μm anisomycin for 30 min. Whole cell lysates were immunoblotted with anti-FLAG antibody and reprobed with anti-SHP2 antibody as a control. D, comparison of amino acid sequences of GRAB serine 169 with different species. The PXSP motif indicates the phospho-MAPK substrate motif.

Journal: The Journal of Biological Chemistry

Article Title: A Phosphoproteomic Screen Identifies a Guanine Nucleotide Exchange Factor for Rab3A Protein as a Mitogen-activated Protein (MAP) Kinase Phosphatase-5-regulated MAP Kinase Target in Interleukin 6 (IL-6) Secretion and Myogenesis *

doi: 10.1074/jbc.M116.769208

Figure Lengend Snippet: Identification of phosphoserine 169 on GRAB as a major phosphorylation site. A, schematic representation of GRAB showing identified MAPK phosphorylation sites. CC, coiled coil. B, wild type GRAB or the indicated GRAB phosphorylation site mutants were co-expressed with vector alone or MKP-5 in 293 cells. Cells were serum-starved for 6 h and stimulated with 5 μm anisomycin (Aniso). Total cell lysates were immunoblotted with pMAPK substrate (Sub) antibody and reprobed with FLAG, MKP-5, or p38 MAPK antibody. C, 293 cells were transfected with FLAG-tagged GRAB, and cells were serum-starved for 6 h and stimulated with 5 μm anisomycin for 30 min. Whole cell lysates were immunoblotted with anti-FLAG antibody and reprobed with anti-SHP2 antibody as a control. D, comparison of amino acid sequences of GRAB serine 169 with different species. The PXSP motif indicates the phospho-MAPK substrate motif.

Article Snippet: Lyophilized peptides were redissolved, and phosphopeptides were enriched by immunoprecipitation using a phospho-MAPK substrate motif antibody (2325, Cell Signaling Technology), which recognizes the conserved MAPK substrate motif (P X S*P or S*P X (R/K)).

Techniques: Plasmid Preparation, Transfection